Utilizing biomarkers to forecast quantitative metabolite concentration profiles in human red blood cells
- Resource Type
- Conference
- Authors
- Yurkovich, James T.; Yang, Laurence; Palsson, Bernhard O.
- Source
- 2017 IEEE Conference on Control Technology and Applications (CCTA) Control Technology and Applications (CCTA), 2017 IEEE Conference on. :961-966 Aug, 2017
- Subject
- Robotics and Control Systems
Biomarkers
Biological system modeling
Predictive models
Biochemistry
Time measurement
Benchmark testing
Red blood cells
Biosystems
Biotechnology
Modeling
- Language
One of the major limitations in making experimental measurements of biological systems is the complexity of the network being investigated. Major efforts have been made to identify a subset of measurements (“biomarkers”) that can be used to provide information about the rest of the system. For red blood cells under cold storage conditions in a blood bank, a set of metabolite biomarkers have been identified that can reliably define the qualitative trend of cellular metabolism. Recently, it was shown that these biomarkers could also be used to train a model that quantitatively predicts the concentrations of other metabolites in the network over a 45 day time course. Here, we extend the utility of these methods by using a linear blackbox model to forecast future values of these concentrations. We show that 57 of the 70 metabolites measured in the red blood cell metabolic network (81%) can be accurately forecasted after 8 days of storage (5 time points) with a global median error of 18.36%. The ability to forecast metabolite profiles by only requiring a subset of measurements for the first few days of storage makes these methods immediately applicable in a clinical setting to assess the metabolic health of stored blood.