In-situ microfabrication of permeation membrane with photo-crosslinkable resin for isolation and culture of individual cells
- Resource Type
- Conference
- Authors
- Arai, F.; Ichikawa, A.; Maruyama, H.; Uchida, T.; Maeda, K.; Yamauchi, R.
- Source
- 18th IEEE International Conference on Micro Electro Mechanical Systems, 2005. MEMS 2005. Micro electro mechanical systems Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on. :822-825 2005
- Subject
- Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Biomembranes
Resins
Microorganisms
Microchannel
Microscopy
Chemical lasers
Fungi
Fluorescence
Ambient intelligence
Systems engineering and theory
- Language
- ISSN
- 1084-6999
We developed a novel technique to investigate individual properties of microorganisms on a chip using in-situ microfabrication of permeation membrane with photo-crosslinkable resin. A cage having vertical permeation membrane was fabricated in situ in the microchannel by providing the photo-crosslinkable resin and curing it in situ by the focused UV irradiation. An arbitrary selected individual cell was transported inside the cage by the newly developed laser micromanipulation method employing a microtool made by the local thermal gelation. The cage was freely and properly constructed at arbitrary position in the microchannel. Since the cage is made of the permeable membrane, we can culture the cells in it and observe their chemical reactions microscopically by providing growth media or reagents. In experiment, we confirmed repeatability of on-demand analysis of isolated individual yeast cells.