A Microfluidic Approach for Studying Responses of Bronchial Smooth Muscle Cells Stretching
- Resource Type
- Conference
- Authors
- Sung, Chun-Hui; Chan, Yao-Fei; Lee, Kang-Yun; Wu, Sheng-Ming; Liu, Cheng-Hsien
- Source
- 2018 IEEE 13th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Nano/Micro Engineered and Molecular Systems (NEMS), 2018 IEEE 13th Annual International Conference on. :36-39 Apr, 2018
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Photonics and Electrooptics
Power, Energy and Industry Applications
Robotics and Control Systems
Performance evaluation
Three-dimensional displays
Nanoelectromechanical systems
Muscles
Printers
Respiratory system
Servomotors
Cell stretching
Microfluidics
bronchial muscle cells
- Language
- ISSN
- 2474-3755
Asthma is a disease about lower respiratory tract inflammation. A global survey shows that there are about 230 million people with asthma. Asthma is an important issue of public health in the developed and developing countries. We propose a stretchable microfluidic bronchial chip made by polydimethylsiloxane (PDMS) and glass for studying the effects of cell stretching in asthma in vitro. The bronchial smooth muscle cells (BSMC) were cultured on microfluidic chip under a continuous stretching and release mimicking human breathing. Stretching device consists of 3D printer components, a servo motor and a control panel. We have demonstrated that regular stretching of cells affect its properties. We performed stretching of cells with 13% elongation in 15 cycle per minute for 2 days. The stretching affected cell alignment of bronchial smooth muscle cells. We observed that direction of cell growth and cell stretching direction are perpendicular to each other.