Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms
- Resource Type
- Conference
- Authors
- Wang, Han-Wei; Chai, Ning; Hu, Song; Dou, Wei; Umulis, David; Wang, Lihong V.; Sturek, Michael; Lucht, Robert; Cheng, Ji-Xin
- Source
- CLEO: 2011 - Laser Science to Photonic Applications Lasers and Electro-Optics (CLEO), 2011 Conference on. :1-2 May, 2011
- Subject
- Photonics and Electrooptics
Lipidomics
Absorption
Microscopy
Atherosclerosis
Biology
Vibrations
- Language
- ISSN
- 2160-8989
We realize vibrational photoacoustic microscopy (VPA) using molecular excitation of overtone vibration and acoustic detection of the resultant pressure transients. We demonstrate 3-D VPA imaging of lipid-rich atherosclerotic plaques and lipid bodies in living Drosophila larvae by exciting 2 nd overtone of the CH bond stretch around 8300 cm −1 . Depth-resolved spectral analysis and a penetration depth of several mm are available.