Centrifugal Step Emulsification Microfluidics Supporting Droplet Digital Loop-Mediated Isothermal Amplification (LAMP) of Sars-Cov-2 N Gene
- Resource Type
- Conference
- Authors
- Shi, Zhi; Dong, Nan; Lai, Xiaochen; Yu, Haixia; Li, Dachao
- Source
- 2021 21st International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) SSolid-State Sensors, Actuators and Microsystems (Transducers), 2021 21st International Conference on. :976-979 Jun, 2021
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Engineered Materials, Dielectrics and Plasmas
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Power, Energy and Industry Applications
Robotics and Control Systems
Resistance
Heating systems
Micromechanical devices
COVID-19
Transducers
Tools
Coronaviruses
Centrifugal Step Emulsification
Droplet Digital LAMP
SARS-CoV-2
- Language
- ISSN
- 2167-0021
Responding to the global crisis of SARS-CoV-2 (COVID-19), the centrifugal step emulsification microfluidics supporting droplet digital Loop-mediated isothermal amplification (LAMP) is presented to quantify the SARS-COV-2 N Gene in this study. The designed centrifugal step emulsification chip with a triangular nozzle shows a robust droplet generation for sample division for LAMP, and the influence by channel flow resistance is experimentally analyzed. In addition, an evaporation-resisted structure is applied to address the reagent loss in the heating process caused by PDMS material. The devices and solution are potential to be a promising tool for the quantification of SARS-CoV-2.