Characterizing Mutant Protein Activators Using Single Molecule Optical Trapping
- Resource Type
- Conference
- Authors
- Ravindranath, Adarsh Lalitha; Alizadehkhaledi, Amirhossein; Khademi, Ali; Omar, Sara Ibrahim; Tuszynski, Jack; Gordon, Reuven
- Source
- 2018 IEEE 13th Nanotechnology Materials and Devices Conference (NMDC) Nanotechnology Materials and Devices Conference (NMDC), 2018 IEEE 13th. :1-4 Oct, 2018
- Subject
- Components, Circuits, Devices and Systems
Biomedical optical imaging
DNA
Optical sensors
Optical imaging
Optical filters
Optical device fabrication
Proteins
plasmonics
near-field optics
optical trapping
optical tweezer
double-nanohole
single molecule optical trapping
protein DNA interaction
DNA unzipping
tumor suppressants
mutant p53 activators
- Language
Optical trapping and characterization of 10 base pairs (bp) hairpin DNA as well as its interaction with tumor suppressing protein (p53), wild and mutant types, using a Double Nano-Hole (DNH) optical tweezer have been previously reported. This paper discusses the application of the above technique in characterizing potential tumor suppressing activators from their interactions with a p53 mutant variant and l0bp hairpin DNA while establishing a comparison with the wild protein-DNA interaction.