Experimental validation of an optofluidic platform for microbial single cell isolation and whole genome amplification for human microbiome applications
- Resource Type
- Conference
- Authors
- Liu, Yuguang; Jeraldo, Patricio; McDonough, Samantha; Jen, Jin; Patel, Robin; Walther-Antonio, Marina; Lambert, Christopher; Gale, Bruce
- Source
- 2017 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Medical Measurements and Applications (MeMeA), 2017 IEEE International Symposium on. :62-66 May, 2017
- Subject
- Engineering Profession
Genomics
Bioinformatics
Valves
Microfluidics
Optical refraction
Optical variables control
Sequential analysis
optofluidics
microfluidics
optical tweezers
single cell
whole genome amplification
microbiome
- Language
Single microbial cell genome sequencing is becoming a powerful tool for the discovery of the hidden genetic information valuable for many medical applications. One of the critical steps in single-cell genome sequencing is the physical isolation of individual cells from a highly diverse heterogeneous population. Amplifying the genome of a single microbial cell is another challenge due to the minute amount of DNA. Efforts have been directed in developing an optofluidic platform integrating advanced microscopy, optical tweezers and microfluidic technology for single cell isolation and genome amplification. Here, we investigate and evaluate the validity of this platform for single microbial cell genome amplification. The successful validation of this approach allows us to perform various single cell studies using this platform.