We report on the performance of a completed 64-pixel positron-sensitive surgical probe using wall-less sphere sources in preparation for clinical testing. The method of making volumetric positron sources by uniformly mixing /sup 18/F with beeswax through a chemical procedure enables us to evaluate the performance of the probe in a more realistic environment. As previously reported, the probe consists of a dual-layer detector and a multianode PMT (Hamamatsu H7546). The first layer consists of an 8/spl times/8 array of thin plastic scintillators, which is 1:1 optically coupled to an 8/spl times/8 array of 2/spl times/ 2/spl times/10 mm/sup 3/ GSO crystals. Each pixel element is optically isolated using CaCO/sub 3/ reflectors. With this dual-layer design and customized 64 channels of electronics (with FPGA), a valid event is a signal in the plastic layer characterized with an overshoot by the pulse-shaping circuit, in coincidence with an annihilation 511 keV gamma in the GSO layer, selected by an energy threshold on the PMT summed dynode signal. Now using wall-less sphere sources we more completely characterize the probe's performance based on a more accurate simulation of the sentinel lymph node surgical environment. The new measurements show that the 64-pixel probe has a sensitivity of /spl sim/20 cps/pixel per /spl mu/Ci/cc using optimal signal selection criteria. The probe is able to identify in 10 seconds a /spl sim/4 mm lesion with a true-to-background ratio of /spl sim/3 at a tumor uptake ratio of /spl sim/8:1.