Microfluidic 3D Hepatic Cultures Integrated with Droplet-Based Bioanalysis Unit for Monitoring Glucose Metabolism Upon Hormonal Stimulation
- Resource Type
- Conference
- Authors
- de Hoyos-Vega, Jose M.; Gonzalez-Suarez, Alan M.; Cedillo-Alcantar, Diana F.; Stybayeva, Gulnaz; Matveyenko, Aleksey; Malhi, Harmeet; Garcia-Cordero, Jose L.; Revzin, Alexander
- Source
- 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) Solid-State Sensors, Actuators and Microsystems (Transducers), 2023 22nd International Conference on. :1982-1985 Jun, 2023
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Robotics and Control Systems
Transducers
Three-dimensional displays
Media
Glucose
System-on-chip
Sensors
Metabolism
Microfluidics
On-chip analysis
Droplet-based assays
Automatization
Organoids
Hepatocytes
Glucose metabolism
- Language
- ISSN
- 2167-0021
We report a microfluidic device that integrates an organoid culture chamber with a bioanalytical unit in which conditioned media from the 140 organoids can be discreetly aliquoted into picoliter droplets for on-chip analysis. This automated microfluidic device enables hormonal stimulation and high temporal resolution of analyte measurements upon cell stimulation without disturbing local concentration gradients in the culture chamber. Glucose consumption of hepatic spheroids upon hormonal stimulation (insulin) was assessed using in-droplet enzymatic colorimetric glucose assay.