Graphene emerged a broad application prospect in the field of electronics due to its atomically thin structure and fascinating conductivity properties. Miniaturization and integration are the roadmaps in electronics such as chips and multi-function sensors. However, the flexible and efficient printing technique of graphene patterns in the vertical direction is still challenging. In this work, we proposed a method to prepare stacked graphene patterns by droplet ejection and micro-pen writing techniques. The conductive graphene lines were printed by the droplet ejection, following by the micro-pen writing insulating cellulose nanotibril (CNF) line and the CNF film. Finally, a microstructure containing two-layer graphene lines was successfully prepared through alternating graphene lines and CNF films printing. Our work provides an accessible method for multi-layer graphene pattern printing, paving the way for the development of integrated electronics.