Amperometric Biosensor Strip With Carbon Nanotube and Ketone Body 3-Hydroxybutyrate Dehydrogenase
- Resource Type
- Periodical
- Authors
- Suzuki, K.; Muguruma, H.; Iwasa, H.; Tanaka, T.; Hiratsuka, A.; Shimidu, T.; Hoshino, Y.; Tsuji, K.; Kishimoto, T.
- Source
- IEEE Sensors Journal IEEE Sensors J. Sensors Journal, IEEE. 23(3):1778-1785 Feb, 2023
- Subject
- Signal Processing and Analysis
Communication, Networking and Broadcast Technologies
Components, Circuits, Devices and Systems
Robotics and Control Systems
Electrodes
Sensors
Biosensors
Gold
Strips
Blood
Biochemistry
3-hydroxybutyrate dehydrogenase (3HBDH)
biosensor strip
carbon nanotube (CNT)
ketone bodies
- Language
- ISSN
- 1530-437X
1558-1748
2379-9153
We propose an amperometric biosensor strip for the detection of ketone body 3-hydroxybutyrate (3HB) in serum. The biosensor strip has a small volume ( $\approx 2 ~\mu \text{L}$ ) capillary chamber. The working electrode consists of carbon nanotubes (CNTs) and 3HB dehydrogenase (3HBDH). The CNTs are dispersed in aqueous media with sodium cholate (SC) surfactant and mixed with HBDH solution. Ultraviolet–visible, electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM) demonstrated that the CNTs were well dispersed in aqueous solution. Any type of CNT enhances the current owing to an enzymatic reaction without an electron transfer mediator. A small amount of HBDH/CNT solution is dipped into a small-area (1.5 mm2) electrode and sealed with a coversheet to make the reaction chamber. The sensor can detect 3HB in human serum within the range of 0.05–4.4 mM, which can cover physiological use. The response time is 15 s.