This paper discusses the impact of manufacturing tolerances on the extracted material parameters for Millimeter-wave (mmWave) antenna-inion-package (AiP/AoP) through simulation. The T-resonator, difference length transmission lines (DLTL) method, and single patch antenna are used to extract the material characteristic of multi -layer substrate. Next, use stacking patch verify the extracted material parameters. Frequency shift is less than +/−100 MHz in the PP thickness +/−15um (+/−25%) process tolerance. Open-stub length variety of per 15um (1.02%) cause about 280MHz frequency shift. Core thickness variety of per 15um (6%) cause about 130MHz frequency shift. Patch size variety of per 25um (1%) cause about 240MHz frequency shift. From the simulated results can find out key point of affect which extracted material parameters. By the optical microscope (OM) measure the key points increase accuracy of extraction material parameters. Finally, this paper uses the extracted material Dk and OM measurement dimensions build simulation model in the ANSYS HFSS, the simulated S11 is close to measured results.