Photobleaching-Insensitive Fluorescence Diagnostics in Skin and Brain Tissue
- Resource Type
- Periodical
- Authors
- Brydegaard, M.; Haj-Hosseini, N.; Wardell, K.; Andersson-Engels, S.
- Source
- IEEE Photonics Journal IEEE Photonics J. Photonics Journal, IEEE. 3(3):407-421 Jun, 2011
- Subject
- Engineered Materials, Dielectrics and Plasmas
Photonics and Electrooptics
Fluorescence
Bleaching
Surgery
Skin
Probes
In vivo
Measurement by laser beam
Fluorescence-guided resection
FGR
5-ALA
PpIX
protoporphyrin
photobleaching
laser-induced fluorescence spectroscopy
LIF
optical diagnostics
tissue optics
skin
brain
dynamic models
system identification
state space models
- Language
- ISSN
- 1943-0655
1943-0647
In this paper, we investigate the possibility of using accurate prediction models for the prediction of protoporphyrin bleaching dynamics to achieve photobleaching-insensitive methods to improve the evaluation of data in an existing clinical fluorescence-guided resection technique. To simulate the scenario, measurements were carried out in vivo on skin of healthy volunteers using a compact fiber-based fluorescence spectroscopy system. We have developed an effective method for the parameterization of sequences of bleaching spectra. We analyze convergence and decay rates with respect to initial conditions and excitation irradiance. We also discuss the consequences and the potential for bleaching-insensitive measurements and their applicability in a few examples from in vivo open brain surgery.