Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis
- Resource Type
- Conference
- Authors
- Imamura, R.; Zhang, Z.; Yoshikawa, T.; Murata, N.; Yamashita, K.; Fukuzawa, M.; Noda, M.; Shimanouchi, T.
- Source
- 2015 IEEE SENSORS SENSORS, 2015 IEEE. :1-4 Nov, 2015
- Subject
- General Topics for Engineers
Fluorescence
Proteins
Principal component analysis
Arrays
Biomembranes
Lipidomics
Optical filters
Biosensor
Liposome
Protein
Array
Cholesterol
Principal Component Analysis
- Language
A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.