Osteoclast-stimulatory transmembrane protein (OC-STAMP) is the responsible protein for osteoclast. Since it poses a danger to bone health, vaccination can be a successful way to prevent the issue. Several immunoinformatics pathways were applied in this recent study analysis to detect effective B-cell epitopes of human OC-STAMP. The physicochemical properties, as well as the transmembrane region of the human osteoclast-stimulatory transmembrane protein, were investigated. The human OC-STAMP homology 3D model was also developed. B-cell graphical epitopes were investigated resorting to BCPREDS, BepiPred, ABCpred, as well as Bcepred. Surface usability, antigenicity, as well as bepipred graphical epitope enumeration were used to verify the results after they were analyzed. Any of the available B-cell three-dimensional models were developed after the epitopes were analyzed. The most convenient B-cell epitopes, according to this research, are “LLSCLLRLLGLLALLLVATAVAVATDHVAFLLAQA”,“VATVCGLLVFLSLGLPPVRCLFALSVP”,“TQRLAQAQATHLLAPPPTWLLQAAQLRLSQEE”. In thefield of osteoclast diagnosis, this study analysis may be veryuseful for future research as well as vaccine designation