An etched tapered single mode optical fiber (SMF) coated with polyaniline (PANI) nanofibers is developed to detect ammonia (NH 3 ) in low concentrations. The SMF is etched with hydrofluoric acid and subsequently tapered using a glass processing workstation. The etched tapered SMF is coated with PANI via spray-coating deposition. This SMF modification significantly enhances the interaction of the evanescent field of the light propagating in the core with the PANI-sensing layer. The modified fiber sensor response is investigated by exposing the sensor to different concentrations of NH 3 over the C-band wavelengths of 1535–1565 nm. Integrating the modified optical fiber with the nanostructured PANI films produces highly sensitive optical sensor that operates at room temperature. The $50~\mu \text{m}$ etched tapered SMF coated with PANI produced response, recovery times, and sensitivity of 58 and 475 s, and 231.5%, respectively, in the C-band range. The limit of detection of the modified fiber sensor was 0.0025%, which is equal to 25 ppm. The developed sensor exhibits good repeatability, reversibility, and selectivity.