Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City, Anhui Province
- Resource Type
- article
- Authors
- WU Zhaochun; HUANG Xu; WANG Jinsheng; QIAN Yezhong; WANG Jin; CHEN Jiwen; LI Yuxin
- Source
- Shanghai yufang yixue, Vol 35, Iss 11, Pp 1058-1062 (2023)
- Subject
- external environment
avian influenza virus
surveillance
Medicine
- Language
- Chinese
- ISSN
- 1004-9231
ObjectiveTo determine the prevalence of avian influenza viruses in the external environment of poultry in Anqing City, Anhui Province, and provide scientific evidence for prevention and control of animal-derived influenza in humans.MethodsA total of 28 farmers’ markets/farms in 10 counties (cities, districts) of Anqing City, Anhui Province, were selected as surveillance sites by simple random sampling strategy. Poultry faeces and other related samples were collected for 6 consecutive weeks. Real-time fluorescence quantitative PCR was used to examine the nucleic acids of influenza A virus. Subtypes H5, H7, and H9 of avian influenza virus were further tested in the positive samples.ResultsA total of 426 specimens were collected, among which 113 tested positive with a positive rate of 26.53%. Among the positive specimens, 104 were determined to be subtype H9, accounting for 92.04%. It did not significantly differ in the positive rate between the main and non-main urban areas (χ20.05) or among the specimens collected in different weeks (χ2=7.57, P>0.05). However, it significantly differed in the positive rate among the specimens collected in the third week and other weeks (χ2=6.89, P