Inhibitory Effect and Mechanism of Lactobacillus reuteri on Inflammation and Apoptosis of Intestinal Epithelial Cells
- Resource Type
- article
- Authors
- CHEN Peng, ZHONG Yuna, YU Liwen, HU Jin, XIE Meiying
- Source
- Shipin Kexue, Vol 45, Iss 6, Pp 113-119 (2024)
- Subject
- lactobacillus reuteri
intestinal health
microrna
inflammation
apoptosis
Food processing and manufacture
TP368-456
- Language
- English
Chinese
- ISSN
- 1002-6630
Objective: To investigate the effect and mechanism of Lactobacillus reuteri on the inflammatory response and apoptosis of intestinal epithelial cells. Methods: Immunofluorescence and enzyme linked immunosorbent assay (ELISA) were used to detect the effect of L. reuteri on the inflammatory response and apoptosis of intestinal epithelial cells. Bioinformatics analysis, real-time polymerase chain reaction (PCR) and the dual-luciferase reporter gene assay were used to analyze downstream miRNA expression under the action of L. reuteri. The downstream target gene of miR-196a and its function were investigated using the dual-luciferase reporter gene assay and Western blot (WB). Results: L. reuteri inhibited inflammatory responses and apoptosis induced by lipopolysaccharide (LPS) in intestinal epithelial cells by up-regulating miR-196a. miR-196a could protect intestinal epithelial cells by targeting the expression of programmed cell death factor 4 (PDCD4). Conclusion: L. reuteri could inhibit the expression of the PDCD4 gene through miR-196a, thereby alleviating the inflammatory response and apoptosis of intestinal epithelial cells.