A quantitative method for detecting meat contamination based on specific polypeptides
- Resource Type
- article
- Authors
- Chaoyan Feng; Daokun Xu; Zhen Liu; Wenyan Hu; Jun Yang; Chunbao Li
- Source
- Animal Bioscience, Vol 34, Iss 9, Pp 1532-1543 (2021)
- Subject
- meat contamination
nano-lc-ms/ms
specific peptide
Zoology
QL1-991
- Language
- English
- ISSN
- 2765-0189
2765-0235
19731337
Objective This study was aimed to establish a quantitative detection method for meat contamination based on specific polypeptides. Methods Thermally stable peptides with good responses were screened by high resolution liquid chromatography tandem mass spectrometry. Standard curves of specific polypeptide were established by triple quadrupole mass spectrometry. Finally, the adulteration of commercial samples was detected according to the standard curve. Results Fifteen thermally stable peptides with good responses were screened. The selected specific peptides can be detected stably in raw meat and deep processed meat with the detection limit up to 1% and have a good linear relationship with the corresponding muscle composition. Conclusion This method can be effectively used for quantitative analysis of commercial samples.