Wei-Hua Wang,1 Ying-Hong Wu,2 Yi-Min Wang,3 Chun-Lei Wang,4 Yun Liu,1 Peng Gao,1 Xiao-Jing Wu,3 Jiao-Qian Ying1 1Department of Medical Affairs, China-Japan Friendship Hospital, Beijing, 100029, People’s Republic of China; 2Department of Nosocomial Infection Control, People’s Hospital of Peking University, Beijing, 100044, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, 100029, People’s Republic of China; 4Laboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, 100029, People’s Republic of ChinaCorrespondence: Jiao-Qian Ying, Department of Medical Affairs, China-Japan Friendship Hospital, No. 2 of Yinghua East Street, Chaoyang District, Beijing, 100029, People’s Republic of China, Tel +86 10 84205608, Fax +86 10 84222965, Email jiaoqian_ying@outlook.comObjective: The present study aims to explore the effects of different numbers of fiberoptic bronchoscopic examinations on the nosocomial infection/colonization of carbapenem-resistant Enterobacteriaceae (CRE).Methods: The data of 129 patients admitted to the intensive care unit of a grade 3A hospital were retrospectively analyzed, and CRE nosocomial infection/colonization situations in patients with fiberoptic bronchoscope application times of 1, 2, 3, and ≥ 4 were statistically analyzed.Results: The incidence of nosocomial infection/colonization of CRE increased significantly when the number of fiberoptic bronchoscopic examinations was ≥ 3.Conclusion: Nosocomial infection/colonization of CRE is highly correlated with an increased number of fiberoptic bronchoscopic examinations.Keywords: nosocomial infection/colonization, carbapenem-resistant Enterobacteriaceae, fiberoptic bronchoscopy, number of inspections