Generation of a CAG-EGFP tagged cell line (KSCBi017-A-2) from human induced pluripotent stem cells using CRISPR/Cas9
- Resource Type
- article
- Authors
- Hyo-Won Han; Young Sam Im; Yong-Ou Kim; Sungwook Kwak; Sun-Jung Cho
- Source
- Stem Cell Research, Vol 75, Iss , Pp 103303- (2024)
- Subject
- Biology (General)
QH301-705.5
- Language
- English
- ISSN
- 1873-5061
Human induced pluripotent stem cells (hiPSCs) have potential use in regerenrative medicine for disease modeling and drug screening studies. The AAVS1 locus has been validated as a stable transgene expression and safe genomic location. Therefore, we inserted the enhanced green fluorescent protein (EGFP) gene into the AAVS1 locus of hiPSCs, using CRISPR/Cas9 genome editing. The results showed that the hiPSCs stably expressed EGFP in pluripotency and differentiated into three germ lineages. Our results strongly indicate that the EGFP-tagged cell line has potential for use in in vivo and in vitro experiments for monitoring cell location and type.