In this work, a malachite green residue detection system for aquaculture monitoring was established. The system includes a new method based on DNA adapter body of malachite green sensor, a handheld electrochemical detector, and the combination of the two, which constitute a set of high performance, convenient and portable malachite green residue detection system. According to the experimental results, the detection of this system is range from 1.0 x 10-3ng . ml-1 to 10ng . mi-1. Hence, the detection system proposed in this paper can meet the accuracy convenience and quick detection of malachite green residue in aquatic products water.