Ali the standard strains and isolates of Lactococcus lactis subsp. lactis biovar diacetylactis were tested for antibiotic sensitivity against 27 antibacterial agents and found that all were sensitive to ampicillin, amoxycillin, bacitracin, chioramphenicol, chlortetracycline, demechlocycline, doxycycline,erythromycin, framycetin, furazolidone, gentamicin, penicillin-B, rifampicin, streptomycin, tetracycline and vancomycin. They showed resistance to amikacin, carbenicillin, metronidazole, nalidixic acid and sulphamethizole. Individual variation among the organisms in resistance to other antibiotics was also noticed.