Ketoreductase Catalyzed (Dynamic) Kinetic Resolution for Biomanufacturing of Chiral Chemicals
- Resource Type
- article
- Authors
- Chenming Huang; Junling Liu; Jiali Fang; Xian Jia; Zhendong Zheng; Song You; Bin Qin
- Source
- Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
- Subject
- ketoreductase
biocatalysis
asymmetric reaction
kinetic resolution
dynamic kinetic resolution
Biotechnology
TP248.13-248.65
- Language
- English
- ISSN
- 2296-4185
Biocatalyzed asymmetric reduction of ketones is an environmentally friendly approach and one of the most cost-effective routes for producing chiral alcohols. In comparison with the well-studied reduction of prochiral ketones to generate chiral alcohols with one chiral center, resolution of racemates by ketoreductases (KREDs) to produce chiral compounds with at least two chiral centers is also an important strategy in asymmetric synthesis. The development of protein engineering and the combination with chemo-catalysts further enhanced the application of KREDs in the efficient production of chiral alcohols with high stereoselectivity. This review discusses the advances in the research area of KRED catalyzed asymmetric synthesis for biomanufacturing of chiral chemicals with at least two chiral centers through the kinetic resolution (KR) approach and the dynamic kinetic resolution (DKR) approach.