Objective: Minimal residual disease (MRD) is considered the greatest prognostic factor in acute lymphoblastic leukemia(ALL). MRD is a valuable tool for anticipating impending relapse and treatment response assessment. The objective ofthe present study was to investigate whether the detection of IgH gene rearrangement using polymerase chain reaction(PCR)-based GeneScan analysis could be a complementary method to monitor MRD along with the quantitative realtimePCR (qPCR).Materials and Methods: In this cross-sectional study, we valued the MRD levels, based on the GeneScanning analysis(GSA), and then compared the data with quantitative real-time polymerase chain reaction at different time points inperipheral blood (PB) samples of adult B-lineage ALL patients (n=35). The specific polymerase chain reaction (PCR)primers for IGH gene FR-1 and fluorescence-labeled J-primer were used and analyzed by capillary gel electrophoresison a sequencer. The results of this study were compared with the previously reported MRD results obtained by the IGHrearrangements allele-specific oligonucleotide (ASO) -qPCR methods.Results: The total concordance rate was 86.7%, with a P