Four bulls each of four breeds (crossbred Holstein Friesian, crossbred Jersey, Vechur and Kasargod Dwarf) used for frozen semen production in Kerala Livestock Development Board were selected to evaluate the effect of breed on the quality of frozen semen. Semen samples of all the bulls (n=16) procured monthly for a period of ten months were subjected to in vitro quality assessment tests. Among the seven quality parameters studied, only progressive motility and cervical mucus penetration distance of post thaw spermatozoa were observed to be significantly (p