Introduction: This study aimed to evaluate the prevalence of hepatitis C virus (HCV) in patients followed up with the diagnosis of diabetic foot infection (DFI) in the Department of Infectious Diseases and Clinical Microbiology of Ege University Faculty of Medicine between 2016-2023. Materials and Methods: Patients who were followed up with the diagnosis of DFI between 2016-2023 were investigated retrospectively for anti-HCV test positivity via electronic patient files. Demographic data, HCV-RNA test results, and treatment status of the patients with positive anti-HCV tests were recorded. The obtained data were entered into the Excel statistical analysis software. Statistical analysis was performed using the Chi-square test, and a p-value of