Protective effect and mechanism of ethanol extract of sesame on myocardial injury in rats with exercise-induced fatigue
- Resource Type
- article
- Authors
- LI Qiang; MAO Yufeng
- Source
- Zhongguo shipin weisheng zazhi, Vol 35, Iss 2, Pp 156-162 (2023)
- Subject
- sesame ethanolic extract
exercise-induced fatigue
antioxidant
gap junctions
energy metabolism
mitochondrial biogenesis
Food processing and manufacture
TP368-456
Nutrition. Foods and food supply
TX341-641
- Language
- Chinese
- ISSN
- 1004-8456
ObjectiveTo study the cardioprotective effect of sesame ethanol extract (SEE) on exercise-induced fatigue (EIF) rats and its effects on myocardial physiological and biochemical parameters.MethodsRats were randomly divided into control group (Control), EIF group, EIF+0.5SEE group, EIF+1SEE group and EIF+2SEE group, with 12 rats in each group. The rats in control group and EIF group were daily intragastrically administered 2 mL of purified water for 14 consecutive days. Rats in EIF+0.5SEE group, EIF+1SEE group and EIF+2SEE group were given 0.5, 1.0 and 2.0 g/kg·BW of SEE daily for 14 consecutive days. Then the EIF rat model was established by treadmill exercise. After exercise, the echocardiographic parameters (LVPWd, LVPWs, LVIDs, EF and FS), serum myocardial injury indexes (LDH, CK and cTnI) and myocardial tissue antioxidant indexes (MDA, SOD, CAT and GSH-Px) of the rats in each group were detected. Myocardial morphology was detected by hematoxylin and eosin (HE) staining. The protein expressions of connexin 43 (Cx43), ATP 5D, phosphorylated AMP-dependent protein kinase (p-AMPK) and AMPK in myocardial tissue were detected by Western blot.ResultsCompared with EIF group, the LVPWd, LVPWs and LVIDs of EIF+0.5SEE group, EIF+1SEE group and EIF+2SEE group decreased, while EF and FS increased significantly (P