ObjectiveTo analyze the prevalence of HCV antibody positive and associated factors among drug users in Dehong Prefecture, Yunnan, and to provide scientific evidence for HCV prevention.MethodsQuestionnaire surveys and serological testing were conducted among 400 drug users continuously selected from four national AIDS sentinel surveillance in Dehong Prefecture between January and July during 2014‒2021.Results11 683 drug users were included. The prevalence of HCV antibody positive was 20.2% overall, and 14.9%, 20.1%, 22.4%, 19.8%, 22.5%, 20.6%, 24.5%, 19.0% from 2014 to 2021, respectively (trend Z=-3.78, P