A recent application of molecular technology in dairy cattle breeding is the identification of the regions of the DNA affecting the production traits. In the present study, the possibility of using the informations of the allele frequency, heterozygosity and PIC of two microsatellite markers and their association with the economically important traits for the selection of crossbred cattle were studied. Both the markers were highly informative, as their PIC values were more than 0.5. Animals with the allele 205 at HUJll77locus had significantly lower milk fat percentage compared to the animals without this allele. The selection against this allele may contribute much in improving the milk fat percentage. For BM4305 locus, the allele 154 had effects on lower milk fat percentage. The selection against this allele may contribute much in improving the milk fat percentage. The animals with the allele 166 had the highest average of milk fat percentage. Selection for this allele will have good impact on higher milk fat percentage