The PCR conditions for RAPD-PCRamplificat ion were standardized for the crossbred cattle of Kerala. Nineteen random oligonucleotide primers were selected based on intensity, clarity and polymorphism of bands from 26 primers for amplification of unrelated DNA samples of crossbred cattle of Kerala. Number of bands obtained for different primers ranged from .2 to 16. The average number of bands produced by different primers ranged from 3.78 ± 0.15 in OPA 4 to 8.95 ± 0.25 in !LO 7127. The size of amplification products ranged from -230 base pairs to 3580 base pairs. The percentage of polymorphism represented by individual primers varied from 66.66 to 900 in different groups. Seven primers namely OPA 2, OPA 4, OPA 97, OPA 98, OPA 20, ILO 9127 and 1L0 876 yielded 900% polymorphism in bands