Weak Value Amplification-Based Biochip for Highly Sensitive Detection and Identification of Breast Cancer Exosomes
- Resource Type
- article
- Authors
- Jingru Zhao; Xiaotian Guan; Sihao Zhang; Zhou Sha; Shuqing Sun
- Source
- Biosensors, Vol 14, Iss 4, p 198 (2024)
- Subject
- weak value amplification (WVA)
biochip
exosomes
breast cancer
Biotechnology
TP248.13-248.65
- Language
- English
- ISSN
- 2079-6374
Exosomes constitute an emerging biomarker for cancer diagnosis because they carry multiple proteins that reflect the origins of the parent cell. The highly sensitive detection of exosomes is a crucial prerequisite for the diagnosis of cancer. In this study, we report an exosome detection system based on quantum weak value amplification (WVA). The WVA detection system consists of a reflection detection light path and a Zr-ionized biochip. Zr-ionized biochips effectively capture exosomes through the specific interaction between zirconium dioxide and the phosphate groups on the lipid bilayer of exosomes. Aptamer-modified gold nanoparticles (Au NPs) are then used to specifically recognize proteins on exosomes to enhance the detection signal. The sensitivity and resolution of the detection system are 2944.07 nm/RIU and 1.22 × 10−5 RIU, respectively. The concentration of exosomes can be directly quantified by the WVA system, ranging from 105–107 particles/mL with the detection limit of 3 × 104 particles/mL. The use of Au NPs-EpCAM for the specific enhancement of breast cancer MDA-MB-231 exosomes is demonstrated. The results indicate that the WVA detection system can be a promising candidate for the detection of exosomes as tumor markers.