Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR
- Resource Type
- article
- Authors
- Xinyun Xu; Xinge Hu; Guodong Ma; Tiannan Wang; Jayne Wu; Xiaojuan Zhu; Guoxun Chen; Ling Zhao; Jiangang Chen
- Source
- Heliyon, Vol 9, Iss 9, Pp e20159- (2023)
- Subject
- fa mutation
Leptin receptor
Tetra-primer amplification-refractory mutation system (ARMS)
Zucker rats
Obesity
Science (General)
Q1-390
Social sciences (General)
H1-99
- Language
- English
- ISSN
- 2405-8440
Due to the genetic mutation (fa) in the gene encoding for leptin receptor, homozygous Zucker rats (fa−/−) develop excessive adiposity and become an experimental animal model in obesity and metabolic-related diseases research. Based on tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), we developed a method to quickly genotype Zucker rats with a mutated fa allele from their wildtype littermates. The three genotypes are clearly discriminated on 2.0% agarose gel. Our method can be used as a reliable tool to set up and maintain the breeding colony in animal facilities as well as assign animals to control and treatment groups based on their genotypes for animal studies.