The objective of this study was to asses the effect of different feeding substrates (FS), harvesting times (HT), and the interaction between FS and HT on the chemical composition of black soldier fly larvae (BSFL). The experiment used a 4 x 2 factorial arrangement with two main factors, FS (T1, T2, T3, T4), and HT (15-d and 20-d). Thus, there were eight treatment combinations, all together with five replications. The results show that there was no interaction (P>0.05) between FS and HT on dry matter (DM), crude protein (CP), crude lipid (CL), phosphorus (P), gross energy (GE), and amino acid contents. The ash content of BSFL grown on T2 media and harvested on day 15 was higher (P