In this contribution, new data concerning algae, bryophytes, fungi and lichens of the Italian flora are presented. It includes new records and confirmations for the algal genera Acetabularia, Nitella, and Nitellopsis for the bryophyte genera Drepanocladus, Fissidens, Hookeria, and Weissia, the fungal genera Alnicola, Arthonia, Cortinarius, Inocybe, Leucoagaricus, Neohygrocybe, and Puccinia and the lichen genera Bacidina, Chaenotheca, Flavoplaca, Gyalecta, Heterodermia, Rinodina, Scytinium, and Squamarina.