A novel -ketoamide reactivity-based two-photon fluorogenic probe for visualizing peroxynitrite in Parkinson’s disease models
- Resource Type
- article
- Authors
- Tao Shao; Xianning Xu; Lan Wang; Yu Shen; Jun Zhao; Huizi Li; Duoteng Zhang; Wei Du; Hua Bai; Bo Peng; Lin Li
- Source
- Journal of Innovative Optical Health Sciences, Vol 16, Iss 04 (2023)
- Subject
- -Ketoamide
two-photon fluorogenic probe
bioimaging
peroxynitrite
Parkinson’s disease
Technology
Optics. Light
QC350-467
- Language
- English
- ISSN
- 17935458
1793-7205
1793-5458
Peroxynitrite (ONOO[Formula: see text] contributes to oxidative stress and neurodegeneration in Parkinson’s disease (PD). Developing a peroxynitrite probe would enable in situ visualization of the overwhelming ONOO− flux and understanding of the ONOO− stress-induced neuropathology of PD. Herein, a novel [Formula: see text]-ketoamide-based fluorogenic probe ( DFlu) was designed for ONOO− monitoring in multiple PD models. The results demonstrated that DFlu exhibits a fluorescence turn-on response to ONOO− with high specificity and sensitivity. The efficacy of DFlu for intracellular ONOO− imaging was demonstrated systematically. The results showed that DFlu can successfully visualize endogenous and exogenous ONOO− in cells derived from chemical and biochemical routes. More importantly, the two-photon excitation ability of DFlu has been well demonstrated by monitoring exogenous/endogenous ONOO− production and scavenging in live zebrafish PD models. This work provides a reliable and promising [Formula: see text]-ketoamide-based optical tool for identifying variations of ONOO− in PD models.