Threshold somatic cell count for delineation of subclinical mastitis cases
- Resource Type
- article
- Authors
- P. V. Jadhav; D. N. Das; K. P. Suresh; B. R. Shome
- Source
- Veterinary World, Vol 11, Iss 6, Pp 789-793 (2018)
- Subject
- discriminate function
mastitis
receiver's operating characteristic curve
somatic cell count
threshold
Animal culture
SF1-1100
Veterinary medicine
SF600-1100
- Language
- English
- ISSN
- 0972-8988
2231-0916
Aim: Somatic cell count (SCC) is the most widely used single reliable indicator of udder health. The present study was carried out with an objective to find the exact threshold of SCC. Materials and Methods: Milk samples collected from a total of 214 Holstein Friesian crossbred dairy animals were subjected to bacterial DNA extraction and SCC estimation by digital PortaCheck. California Mastitis Test and polymerase chain reaction based on amplification of organism using reported primers were performed to diagnose subclinical mastitis. Receiver's operating characteristic (ROC) curve analysis and discriminate function analyses were performed using SPSS 18 software. Results: ROC curve analysis represented that the area under the curve was 0.930 with the standard error of 0.02. Results indicated that 93% of the case could be correctly predicted as mastitis infected using SCC as a marker (p