Effect of nutrients and fermentation conditions on the production of biosurfactants using rhizobacteria isolated from fique plants
- Resource Type
- article
- Authors
- Aura M. Pedroza-Rodríguez; Refugio Rodríguez-Vásquez; Leonardo Sastoque-Cala; Alba M. Cotes-Prado
- Source
- Universitas Scientiarum, Vol 15, Iss 3, Pp 251-264 (2010)
- Subject
- Pseudomonas fluorescens
biosurfactant
rhamnose
emulsification index
soybean oil
Science (General)
Q1-390
- Language
- English
Spanish; Castilian
- ISSN
- 0122-7483
2027-1352
To isolate biosurfactant-producing microorganisms from the rhizosphere of fique and to select the best genus to evaluate theeffect of nutritional and fermentation conditions on the production of rhamnolipids. Materials and methods. Rhizospheric soil wassampled in three areas of Cauca. The best genus was selected for the experimental designs (Plackett Burman and 22 factorial) and to find theproduction conditions for the growth kinetics at an Erlenmeyer flask scale. Results. Isolates from the rhizosphere of fique plants were fromgroups (or genera) of Bacillus, Pseudomonas and Actinomycetes, being Pseudomonas the more responsive in preliminary testing foremulsification. From the results of the experimental designs and the kinetics of production, we found that rhamnose synthesis associatedwith rhamnolipids (3.2 g/l) and emulsification (68% EC24) was significantly favored (p