Effects of sample handling on the stability of interleukin-6 in patients with breast neoplasms
- Resource Type
- research-article
- Authors
- Wang, Yunxiu; Wang, Baiye; She, Lanhui; Wang, Jijuan; Chen, Yingting; Huang, Di; Han, Guang; Lu, Miaolian; Wu, Xiaobin; Wan, Zemin; He, Min; Ke, Peifeng; Huang, Xianzhang; Liu, Hongcan
- Source
- Journal of Laboratory Medicine. 46(2):115-119
- Subject
- interleukin-6 (IL-6)
pre-analytical
stability
storage time
temperature
- Language
- English
- ISSN
- 2567-9449
2567-9430
Objectives To investigate the influence of preservation methods and processes on the plasma interleukin-6 (IL-6) stability. Methods Lithium-heparin plasma was collected from female patients: 32 female patients with invasive breast neoplasms and 20 healthy females. Each sample was divided into three tubes. Samples were stored at different temperatures or at different times. The concentration of IL-6 was detected. Results IL-6 levels in patients were not altered significantly compared to the control group after storage at 4 °C or 25 °C for 12 h. However, IL-6 levels were significantly higher compared to controls (p Conclusions Concentrations of IL-6 in breast neoplasms samples significantly change under different storage conditions. Pretreatment needs to be standardized for blood sample handling procedure. Comparison of different storage conditions of IL-6 levels may not be reliable.