通过以香豆素为唯一碳源和能源的培养基进行菌株筛选,从土壤中获得一株能够降解黄曲霉毒素的菌株Adt.16S rDNA序列及6-磷酸葡萄糖脱氢晦FGD基因序列用以对菌株进行分子生物学鉴定,薄层层析(TLC)和ELISA法用来检测该菌株对香豆素和黄曲霉毒素B1(AFB1)的降解活性.研究结果表明,菌株Adt的16S rDNA及FGD序列与食醚红球菌(Rhodococcus aetherivorans)的相应序列高度同源,为一株食醚红球菌(R.aetherivorans),TLC 及ELISA结果显示其对香豆素和AFB1均有降解活性,其含有的FDR家族的还原酶很可能直接发挥这一活性.
In this paper,a strain named Adt was isolated from soil.The DNA sequences of 16S rDNA and F420-dependent glucose 6-phosphate dehydrogenase (FGD) were utilized to identify the strain.TLC and ELISA were analyzed for the degradation of coumarin and AFB1.The DNA sequence alignments of the Adt by 16S rDNA and FGD showed that it had high identity to Rhodococcus aetherivorans.The results of TLC and ELISA showed that the strain Adt could degrade coumarin and AFB 1,and a reductase belonged to FDR family which isolated from its genome was probably responsible for part of this activity.