目的:研究血清微管解聚蛋白1(STMN1)以及组织蛋白酶D(Cath-D)含量与宫颈癌病灶浸润性生长的相关性.方法:选择在本院妇科接受手术切除的宫颈癌患者和子宫肌瘤患者,分别纳入研究的恶性组和良性组;术前采集血清并测定STMN1 、Cath-D的含量;术后采集恶性组患者的宫颈癌病灶组织和良性组患者的正常宫颈组织,测定STMN1 、Cath-D 、侵袭基因、侵袭信号分子的表达量.结果:恶性组血清中STMN1 、Cath-D的含量以及病灶组织中STMN1 、Cath-D的mRNA 表达量显著高于良性组且恶性组患者血清中STMN1 、Cath-D的含量与病灶组织中STMN1 、Cath-D的mRNA表达量呈正相关;恶性组患者病灶组织中RACK1 、Twist 、MMP9 、mTOR 、S6K1 、Wnt 、β-catenin的mRNA 表达量显著高于良性组,E-cadherin 、GRIM19 、GSK-3β的 mRNA 表达量显著低于良性组且恶性组血清中ST-MN1 、Cath-D的含量与病灶组织中RACK1 、Twist 、MMP9 、mTOR 、S6K1 、Wnt 、β-catenin的mRNA表达量呈正相关,E-cadherin 、GRIM19 、GSK-3β的mRNA表达量呈负相关.结论:宫颈癌患者血清中异常升高的STMN1 、Cath-D能够对病灶浸润性生长做出评估.
Objective :To study the correlation of serum stathmin 1 (STMN1) and cathepsin D (Cath-D) levels with the invasive growth of cervical cancer .Methods :The patients with cervical cancer and those with uterine fibroids who underwent surgical resection in the Second Peopleˊs Hospital of Yichang between January 2013 and November 2017 were selected and en-rolled in the malignant group and the benign group of the study respectively ;the serum was collected before operation to detect the contents of STMN1 and Cath-D ;the cervical cancer lesion tissues were collected from the malignant group and the normal cervical tissues were collected from the benign group to measure the expression of STMN 1 ,Cath-D ,invasion genes and inva-sion signal molecules .Results :STMN1 and Cath-D contents in serum as well as STMN1 and Cath-D mRNA expression in le-sion tissue of malignant group were significantly higher than those of benign group ,and serum STMN1 and Cath-D contents of malignant group were positively correlated with STMN1 and Cath-D mRNA expression in lesion tissue ;RACK1 ,Twist , MMP9 ,mTOR ,S6K1 ,Wnt and β-catenin mRNA expression in lesion tissue of malignant group were significantly higher than those of benign group whereas E-cadherin ,GRIM19 and GSK-3β mRNA expression were significantly lower than those of be-nign group ,and serum STMN1 and Cath-D contents of malignant group were positively correlated with RACK1 ,Twist , MMP9 ,mTOR ,S6K1 ,Wnt and β-catenin mRNA expression in lesion tissue ,and negatively correlated with E-cadherin , GRIM19 and GSK-3β mRNA expression .Conclusion :The abnormally elevated STMN1 and Cath-D in serum of patients with cervical cancer can evaluate the invasive growth of the lesion .