目的:探讨细菌生物膜对感染机体Th17细胞的刺激作用。方法:将临床肺泡灌洗液标本中分离到的细菌测定其生物膜形成能力;检测健康人群和生物膜细菌感染人群以及非生物膜细菌感染人群的外周血Th17细胞表达率和IL-17细胞因子水平以及生物膜细菌感染人群的肺泡灌洗液中Th17细胞表达率和IL-17细胞因子水平,用SPSS17.0统计学软件分析上述数据的差异。结果:生物膜细菌感染人群和非生物膜细菌感染人群以及健康对照人群外周血Th17细胞表达率和IL-17细胞因子水平分别是:(0.59±0.18)%和(108.8±20.5) pg/ml、(0.58±0.18)%和(100.1±20.7) pg/ml、(0.55±0.17)%和(100.0±21.4)pg/ml,相互之间没有统计学差异,P>0.05;生物膜感染人群肺泡灌洗液中 Th17细胞表达率和IL-17细胞因子水平分别是(1.37±0.34)%和(157.4±30.8)pg/ml,非生物膜感染人群肺泡灌洗液中 Th17细胞表达率和IL-17细胞因子水平分别是(1.11±0.21)%和(136.2±24.3)mg/ml,相互之间有统计学差异,P<0.05。结论:细菌生物膜能够引起机体感染局部Th17细胞表达增高以及IL-17细胞因子升高,这可能是生物膜引起感染慢性化损伤的机制。
Objective:To study of change of Th17 cell and IL-17 caused by bacteria biofilm after infection.Methods: To detect biofilm formation ability of bacteria isolated from bronchus alveolus washing-water of patients;to detect expressing ratios of Th17 cells and IL-17 level in peripheral blood of patients and healthy person;to detect expressing ratios of Th17 cells and IL-17 level in bronchus alveolus washing-water of patients;to analyze the above data using SPSS17.0 software.Results: Expressing ratios of Th17 cells and IL-17 level in peripheral blood of patients infected by producing biofilm and non-producing biofilm bacteria and healthy person are respectively(0.59±0.18)%and(108.8±20.5)pg/ml,(0.58±0.18)%and(100.1±20.7)pg/ml,(0.55±0.17)% and(100.0± 21.4)pg/ml,they were not different among data from patients and healthy person,P>0.05,expressing ratios of Th17 cells and IL-17 level in bronchus alveolus washing-water of patients infected by producing biofilm and non-producing biofilm bacteria were respective (1.37±0.34)%,(157.4±30.8)pg/ml and(1.11±0.21)%,(136.2±24.3)mg/ml,the data between patients infected by producing biofilm and non-producing biofilm bacteria were distinctly different, P<0.05.Conclusion: Bacteria biofilm can cause Th17 cell expressing ratio and IL-17 level to become high in infected part,this may be mechanism that infection become chronic.