目的:建立亚玛熏药散中木香烃内酯的含量测定方法.方法:采用高效液相色谱法,色谱柱为ZORBOX SB-C18 5 μm4.6 mm×250 mm;流动相为乙腈:水(50∶50)洗脱;流速:0.8 mL/min;柱温:35 ℃;检测波长:225 nm.结果:木香烃内酯在8.59~85.91 μg/mL的浓度范围内线性关系良好,r=0.9999,回收率为97.4%.结论:该方法经方法学验证准确可行,可用于亚玛熏药散的质量控制.
Objective Aim to establish a method for the deter mination of Costunolide in Yama fumigation powder.Methods High performance liquid chromatography was used.The column was ZORBOX SB-C18 5 μm 4.6 mm × 250 mm,the mobile phase was ace-tonitrile:water(50∶50),the flow rate was 0.8 mL/min,the column temperature was 35 ℃,and the detection wavelength was 225 nm.Results the linear relationship of Costunolide was good in the range of 8.59-85.91 μg/mL,r=0.9999,the recovery rate was 97.4%.Conclusion The method is proved to be accurate and feasible by methodology,and can be used for the quality control of Yama fumigation powder.