背景:miR-10b能够调控乳腺癌干细胞的相关特性, 乙醛脱氢酶1是最重要的乳腺癌干细胞标记物之一, 二者在乳腺癌细胞中的相互作用尚需进一步研究.目的:探讨miR-10b对乳腺癌MCF-7细胞中乳腺癌干细胞标记物乙醛脱氢酶1 mRNA和蛋白表达的影响.方法:常规培养乳腺癌MCF-7细胞株, 分别将miR-10b模拟物或其阴性对照转染进入乳腺癌MCF-7细胞;转染48 h, 通过实时荧光定量PCR实验和Western blot实验检测乙醛脱氢酶1 mRNA和蛋白的表达.结果与结论: (1) 将miR-10b模拟物转染进入MCF-7细胞之后, miR-10b在MCF-7细胞株中的表达显著高于对照组 (P=0.003 47); (2) miR-10b过表达之后显著上调了MCF-7细胞株中乙醛脱氢酶1 mRNA和蛋白的表达 (P=0.009 54和P=0.003 11); (3) 结果表明, 上调miR-10b表达能够诱导乙醛脱氢酶1阳性乳腺癌干细胞的产生, 从而为miR-10b调控乳腺癌细胞侵袭、转移提供新的研究依据.
BACKGROUND: MicroR-10 b can regulate the characteristics of breast cancer stem cells, and acetaldehyde dehydrogenase 1 (ALDH1) is one of the most important markers of breast cancer stem cells. The interaction between them in breast cancer cells needs further explorations. OBJECTIVE: To investigate whether over-expression of microRNA-10 b affects ALDH1 mRNA and protein levels in human breast cancer MCF-7 cells. METHODS: hsa-miR-10 b mimics or its negative control was transfected into breast cancer MCF-7 cell line. At 48 hours after transfection, the mRNA and protein expression of ALDH1 in the cells was detected using real-time RT-PCR and western blot assays, respectively. RESULTS AND CONCLUSION: Over-expression of microR-10 b was found in the MCF-7 cell line transfected with hsa-miR-10 b mimics, which was significantly higher than that in the control group (P=0.003 47). Both of ALDH1 mRNA and protein levels were up-regulated in the MCF-7 cell line overexpressing microR-10 b, as compared with the control group (P=0.009 54 and P=0.003 11, respectively). To conclude, over-expression of microR-10 b induces the ALDH1 mRNA and protein expression in the breast cancer MCF-7 cell line, providing new evidence that microR-10 b regulates the invasion and metastasis of breast cancer cells.