目的:建立同时测定余甘子果核中没食子酸(Gallic acid)、柯里拉京(Corilagin)、没食子基儿茶素没食子酸酯((-)-Gallocatechin gallate,GCG)、诃黎勒酸(Chebulagic acid)、1,2,3,4,6-O-五没食子酰葡萄糖(1,2,3,4,6-Pentagalloylglucose,PGG)、鞣花酸(Ellagic acid)6 种鞣质类成分的 HPLC 方法.方法:采用 Agilent Zorbax C18色谱柱(250 mm ×4.6 mm,5μm),流动相为甲醇(A)-0.1%磷酸(B),梯度洗脱:流速:1.0 mL·min-1;检测波长:270 nm;柱温:25℃.结果:余甘子果核中6个成分分离度良好,没食子酸、柯里拉京、GCG、诃黎勒酸、PGG、鞣花酸的线性范围分别在 0.0836~0.5021 μg、0.0652~0.3912 μg、0.0256~0.1536 μg、0.0710~0.4260 μg、0.0916~0.5496 μg、0.2784~1.6704 μg;线性方程分别为 Y=2753.9X+8.6667、Y=1013.1X+107.69、Y=4517.3X-24.433、Y=3087.8X+29.947、Y=1097.5X-20.267、Y=7588.6X-364.58范围内与峰面积呈良好的线性关系;精密度、重复性良好,RSD均小于5%;在室温条件下12 h内稳定;平均加样回收率在97.05%~102.30%之间(RSD为1.10%~1.90%).结论:该方法简便、准确,重复性好,可用于余甘子果核中没食子酸、柯里拉京、GCG、诃黎勒酸、PGG、鞣花酸6种成分的同时测定.为余甘子果核的质量研究提供一定的理论依据.
Objective To establish the simultaneous determination of Gallic acid,Corilagin,Gallocatechin gallate((-)-Gal-locatechin gallate,HPLC method for six tannins including GCG,Chebulagicacid,1,2,3,4,6-O-pentagalloylglucose(1,2,3,4,6-pentagalloylglucose(PGG)and Ellagic acid.Methods The chromatography was performed on Agilent Zorbax C18 column(250 mm × 4.6 mm,5 μm)with mobile phase consisted of methanol(A)-0.1%phosphoric acid(B)and gradient elution at the flow rate of 1.0 mL·min-1.Detection wavelength:270 nm;Column temperature:25 ℃.Results The separation degree of 6 compo-nents in the seed of Emellia mongolica was good.The linear ranges of gallic acid,Corritin,GCG,harillic acid,PGG and ellagic acid were 0.0836~0.5021 μg,0.0652~0.3912 μg,0.0256~0.1536 μg,0.0710~0.4260 μg,0.0916~0.5496 μg,0.2784~1.6704 μg,respectively.The linear equations are Y=2753.9X+8.6667,Y=1013.1X+107.69,Y=4517.3X-24.433,Y=3087.8X+29.947,Y=1097.5X-20.267,Y=7588.6X-364.58 in the range of good line with the peak area Sexual relations;The precision and repeatability were good with RSDS less than 5%.Stable within 12 h at room temperature;The average recoveries were 97.05%~102.30%(RSD 1.10%~1.90%).Conclusion The method is simple,accurate and reproducible,and can be used for simultaneous determination of gallic acid,corritin,GCG,Harillic acid,PGG and ellagic acid in the fruit core of Emellia japonica.To provide a certain theoretical basis for the quality research of the fruit kernel of Phyllanthus emblica.