根据已公布液泡膜 H+-PPase基因家族同源序列保守区设计简并引物,克隆出海滨雀稗中PvVP1基因的中间序列,然后用快速扩增cDNA末端(rapid amplification of cDNA end,RACE)方法,从海滨雀稗中克隆到PvVP15′cDNA序列。该序列ORF长1605 bp,编码535个氨基酸,其编码序列、氨基酸序列与玉米相应基因的同源性分别为93%和99%。
A pair of degenerated primers were designed based on previously cloned homologous cDNA seg-ments.The middle segment of PvVP1 was cloned,then the PvVP1 5 ’cDNA sequence also obtained using 5 ’ RACE.The open reading frame (ORF)of this sequence was 1605 bp,encoding 534 amino acids.The codon and amino acid sequences of the 5’ends of thePvVP1 gene shared 93% and 99% similarity,respectively,with those of Zeamays.