为红托竹荪优良菌种的选育和开发利用提供参考,利用ITS和ISSR分子标记对18个贵州红托竹荪种质的遗传多样性进行分析。结果表明:1)18个红托竹荪菌株的ITS序列长度为611~621 bp,GC碱基的含量为53.9%~54.68%,相似性为99.9%~100%。2)在遗传相似度为0.77时,将18个红托竹荪菌株分为2个类群。第Ⅰ类群包含13个菌株,分别为3个栽培菌株(ZS017,ZS036,ZS038)和10个野生菌株(YZS010-1,YZS010-2,YZS012,YZS018,YZS033,YZS035,YZS039,YZS041,YZS044,YZS046);第Ⅱ类群包含5个野生菌株(YZS040,YZS043,YZS045,YZS047,YZS052)。结论,贵州18个红托竹荪种质的亲缘关系较近,没有明显的地域分布特点,其遗传背景差异较小。
To provide a reference for breeding and utilizing D.rubrovolvata,the authors analyzed the genetic diversity of D.rubrovolvata strains isolated from Guizhou by ITS and ISSR.Results:1 )ITS sequence length of 18 D.rubrovolvata strains was 611~621bp,GC base content was 53.9%~53.9% and the similarity was 99.9%~100%.2)The clustering analysis of ISSR showed that these strains could be divided into two groups at a similarity level of 0.77.GroupⅠcontained 13 strains,of which there were three cultivated strains(ZS017,ZS036,ZS038)and 10 wild strains(YZS010-1,YZS010-2,YZS012, YZS018,YZS033,YZS035,YZS039,YZS041,YZS044,YZS046).GroupⅡ contained five wild strains (YZS040,YZS043,YZS045,YZS047,YZS052 ).In conclusion,the genetic relationship of these 18 strains was close,and there was not obvious regional distribution characteristics.The genetic background was of little difference.