As a new concept having emerged in last few years,the"deep eutectic solvents"(DESs)effect inte-grated into the imprinting technology inevitably exposes design limitations of stimuli-responsive molec-ularly imprinted polymers(MIPs),as well as inadequate analysis of the adsorption performance of MIPs.Herein,a simple yet defined N-isopropylacrylamide/(3-acrylamidopropyl)trimethylammonium chloride(NIPAM/APTMAC)binary DESs system was proposed to prepare intelligent MIPs with thermo-sensitivity.Accordingly,magnetic and thermo-responsive MIPs based on functional monomers-derived DESs(TM-DESs-MIPs1)were synthesized,revealing DESs effect-regulated affinity/kinetics for the enhanced adsorp-tion capability,eco-friendly thermo-regulated elution for high release efficiency,and simple magnetic separation,along with superior selectivity to rhein(RH)and good regeneration ability.TM-DESs-MIPs1 were utilized to extract RH from Cassiae semen samples coupled with high performance liquid chro-matography(HPLC),yielding satisfactory recoveries(79.47%-110.82%)and low limits of detection(LOD)(16.67 μg/L).Another two kinds of MIPs adopting the thermo-responsive moiety-derived DESs effect strat-egy further demonstrated great applicability of such intelligent MIPs for analyses of complicated samples.