We evaluated the biomass and ergosterol con-tent of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid (SA) and methyl jasmonic acid (MeJA) supplementation. The optimal SA concentration was 100μmol?L-1, where the highest ergosterol content of 2.33 mg?g-1 was obtained following 6-day cultivation with 100μmol?L-1 SA supplementation, and which was sig-nificantly higher than the unsupplemented control (p<0.01). Following 4-day supplementation with 50μmol?L-1 MeJA, the highest ergosterol content obtained was 1.988 mg?g-1, which was 25.8%higher than the unsupplemented control. Our data indicate that SA and MeJA supplementation improves ergosterol content in H. erinaceus mycelium.