研究了川赤芍总苷及其主成分芍药苷对痤疮相关炎症的抑制作用.通过制备并基于高效液相色谱分析川赤芍总苷主活性成分含量;建立痤疮丙酸杆菌(Cropionibacterium acnes,C.acnes)诱导THP-1细胞炎症模型;采用CCK8法检测细胞相对活力,ELISA法快速检测细胞IL-1β、IL-8的分泌量,并用Western blot法验证.结果表明:在100 μg/mL质量浓度下川赤芍根提取物能显著抑制C.acnes刺激引起的炎症因子IL-1β、IL-8(p<0.01).从川赤芍根提取物中提取的川赤芍总苷,在相同浓度下对炎症因子IL-1β抑制率提高了20%.川赤芍总苷中芍药内酯苷、芍药苷、苯甲酰芍药苷含量分别为0.64%,26.3%,1.17%.其中芍药苷的含量最高,且对C.acnes刺激引起的炎症因子IL-1β抑制效果显著(p<0.01).此外,芍药苷还能降低C.acnes刺激引起的活性氧和乳酸脱氢酶.综上所述,川赤芍总苷及其主成分芍药苷对改善C.acnes引起的皮肤炎症具有潜在的治疗价值.
In this study,the anti-inflammatory capacity of total paeoniflorin was evaluated by establishing an inflammatory model of Cropionibacterium acnes(C.acnes)-induced THP-1 cells and exploring its main active components for anti-inflammation.The results show that at a concentration of 100 μg/mL,the root extract of Paeonia veitchii significantly inhibits the inflammatory factors IL-1β and IL-8 induced by C.acnes(p<0.01).Total glucoside of Paeonia veitchii from the root extract of Paeonia veitchii increases the inhibition rate of inflammatory factor IL-1β by 20%at the same concentration.The contents of albiflorin,paeoniflorin and benzoyl paeoniflorin in the total glucoside of Paeonia veitchii are 0.64%,26.3%and 1.17%,respectively.Among them,paeoniflorin has the highest content and the most significant inhibition of inflammatory factor IL-1β induced by C.acnes.In addition,paeoniflorin reduces the reactive oxygen species and lactate dehydrogenase induced by C.acnes.In conclusion,the total glucoside extract of Paeonia veitchii and its main active ingredient paeoniflorin have potential therapeutic value in improving the skin inflammation caused by C.acnes.